Coding

Part:BBa_K2740000

Designed by: Zhaoqing Hu   Group: iGEM18_Nanjing-China   (2018-09-16)


Pnif-nifB-nifH-nifD-nifK-nifE-nifN-nifX-hesA-nifV

This part is a nitrogen fixation cluster including nifB, nifH, nifD, nifK, nifE, nifN, nifX, hesA, nifV under control of a σ70 (σA)-dependent promoter located upstream of nifB which we call it PnifB. And expression of this gene cluster is negatively regulated in response to oxygen and fixed nitrogen.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None